Vitamin D deficiency is one of the most commonly discussed nutritional problems in Pakistan, yet the cost of finding out your level varies widely from one laboratory to another. A single blood test can cost a few hundred rupees at one laboratory and several thousand at another, and the test may be listed under different names: Vitamin D, Vitamin D Total, or 25 OH Vitamin D. This comparison puts the major laboratories side by side, using each laboratory’s own price list on All Lab Tests, as listed in November 2026. Prices may vary by branch or city, so confirm the current rate with the laboratory before you visit.
Vitamin D Test Prices Compared
Each row below shows the test exactly as it is named on the laboratory’s list. Where a laboratory lists more than one vitamin D variant, each variant would be shown separately; in this comparison, each laboratory below lists a single vitamin D entry, so there is one row per laboratory.
| Lab | Test (as listed) | Price (PKR) | Notes |
|---|---|---|---|
| Chughtai Lab | Serum 25-OH Vitamin D | 4,200 | Official Chughtai Lab rate; the All Lab Tests list shows PKR 3,700, so the official rate is used here (see note below). |
| Dow Lab | Vitamin D Total (25-OH) D2+D3 | 2,060 | Measures both D2 and D3 forms, as listed. |
| Khan Lab | Vitamin D3 | 1,800 | As listed. |
| Alnoor Diagnostic Centre | Vitamin D with Graph | 500 | Lowest price in this comparison, as listed; confirm with the laboratory what this entry includes. |
| Aga Khan University Hospital | – | Price not publicly listed, confirm with the hospital | Individual test prices are not published publicly by AKUH. |
Note on the Chughtai Lab price: The All Lab Tests Chughtai list shows Serum 25-OH Vitamin D at PKR 3,700, but Chughtai Lab’s own official test search lists Serum 25-OH Vitamin D (code 5050) at PKR 4,200. Following the same rule as our CBC price comparison and our dengue test price comparison, the official rate of PKR 4,200 is used in the table above.
Price Summary
The summary below uses only the prices in the table above.
- Price range: PKR 500 (Alnoor Diagnostic Centre) to PKR 4,200 (Chughtai Lab, official rate).
- Lowest listed price: Alnoor Diagnostic Centre, Vitamin D with Graph, PKR 500.
- Highest listed price: Chughtai Lab, Serum 25-OH Vitamin D, PKR 4,200 (official rate; PKR 3,700 on the All Lab Tests list).
- Middle of the range: Khan Lab Vitamin D3 at PKR 1,800 and Dow Lab Vitamin D Total (25-OH) D2+D3 at PKR 2,060.
No vitamin D entry was found on the price lists of Dr. Essa Laboratory, Islamabad Diagnostic Centre (IDC) or Ibn-E-Sina Clinical Laboratory, so those laboratories have no row in the table. This does not mean they do not offer the test; it means no vitamin D price was findable on their lists on this site, and you would need to ask them directly. Aga Khan University Hospital is included without a number because its individual test prices are not publicly listed.
What Does a Vitamin D Test Measure?
Most vitamin D tests measure 25-hydroxy vitamin D, written as 25(OH)D, in the blood. This is the main storage form of vitamin D in the body, and it reflects vitamin D obtained from sunlight, food and supplements over the preceding weeks. That is why doctors usually ask for the 25-hydroxy test, rather than the active hormone form, when they want to check someone’s vitamin D status. Some laboratories report total 25(OH)D, which combines the D2 and D3 forms, while others list a D3 test specifically; the test names in the table above reflect exactly what each laboratory lists.
Why Do Doctors Order a Vitamin D Test?
Doctors commonly consider vitamin D testing for general reasons such as bone pain, muscle weakness, tiredness or fatigue, frequent illness, or a higher risk of deficiency, for example, limited sun exposure, a diet low in vitamin D, or conditions that affect how the body absorbs nutrients. This is general information only. Vitamin D deficiency often causes no obvious symptoms at all, and symptoms such as fatigue and bone pain have many possible causes, so testing, and what to do about a result, is a decision for your doctor, based on your own history and examination.
Vitamin D Levels: A General Reference Guide
Vitamin D results are usually reported in nanograms per millilitre (ng/mL) or nanomoles per litre (nmol/L). As a general reference only, many laboratories use bands along these lines: below 20 ng/mL is commonly described as deficient, 20 to 30 ng/mL as insufficient, and 30 to 100 ng/mL as sufficient. These bands are a general guide, not a diagnosis. Reference ranges and units vary by laboratory and by the method used, so the range printed on your own report is the one that applies to you, and your doctor interprets the result together with your symptoms, diet, sun exposure, medicines and other test results. Do not start or change a supplement dose based on a single number without discussing it with your doctor, because very high vitamin D levels can also be harmful.
Sunshine, Diet and Vitamin D: General Lifestyle Information
The body makes vitamin D when skin is exposed to sunlight, and vitamin D is also found in foods such as oily fish, egg yolks and fortified milk or cereals. This is general lifestyle information only, not a prescription. How much vitamin D a person makes from sunlight depends on skin tone, the time of day and year, how much skin is exposed, and how much time is spent outdoors, and sun exposure must be balanced against the risk of sun damage. If you are concerned about your vitamin D level, discuss diet, sunlight and supplements with your doctor rather than relying on general advice alone.
How to Prepare for a Vitamin D Test
Preparation is usually simple, and this is general information only. A vitamin D test is a blood test, and fasting is not normally required, but always follow the instructions given by your doctor or the laboratory, because requirements can differ. Bring your doctor’s test slip, a form of identification, and a list of any supplements or medicines you take, since these can be relevant to interpreting the result. Home sample collection is offered by some laboratories; ask the laboratory whether it is available in your area and whether it carries an extra charge, as that is not included in the prices above. If your doctor has also asked for a blood count, see our CBC test price comparison for CBC prices at the major laboratories.
How to Get Your Vitamin D Test Report Online
Most major laboratories in Pakistan offer online reports through their websites or patient portals. You usually need the receipt number, MR number or mobile number used at the time of sample collection, and the report is downloaded as a PDF or viewed on the laboratory’s portal. The following All Lab Tests pages explain how to access reports for each laboratory: Chughtai Lab online report, Dr. Essa Lab online report, IDC online report, Dow Lab online report, Ibn-E-Sina online report, Alnoor online report, and Aga Khan Lab online report. Report availability time is not stated here because it varies by test, laboratory and workload; if a report is delayed, contact the laboratory with your receipt details.
How to Choose a Laboratory for a Vitamin D Test
No single laboratory is declared best here, because the right choice depends on your situation. Useful factors to weigh are: the exact test your doctor asked for, and whether the laboratory lists it as 25-OH Vitamin D, Vitamin D Total or Vitamin D3; the price of that exact test; how close the branch or collection point is, or whether home collection is available; how you will receive the report; and whether your doctor prefers results from the same laboratory over time, so repeat tests can be compared. A lower price alone does not make one test the same as another if the test names or methods differ, and a higher price does not by itself mean a better result. Confirm the test name, price and sample requirements with the laboratory before you go.
Frequently Asked Questions
What is the 25-hydroxy vitamin D test?
It is the blood test most often used to check vitamin D status. It measures 25-hydroxy vitamin D, the main storage form of the vitamin, which reflects vitamin D from sunlight, food and supplements over recent weeks.
How much does a vitamin D test cost in Pakistan?
In this comparison, prices range from PKR 500 at Alnoor Diagnostic Centre to PKR 4,200 at Chughtai Lab (official rate), with Dow Lab at PKR 2,060 and Khan Lab at PKR 1,800. Prices are as listed in November 2026 and may vary by branch or city.
What do vitamin D deficiency, insufficiency and sufficiency mean?
As a general reference, below 20 ng/mL is commonly described as deficient, 20 to 30 ng/mL as insufficient, and 30 to 100 ng/mL as sufficient. Ranges and units vary by laboratory, and your doctor interprets your result in context, so the range on your own report is the one that matters.
Do I need to fast before a vitamin D test?
Fasting is not normally required for a vitamin D test, but follow the instructions from your doctor or the laboratory, as requirements can differ by laboratory.
Why is there no price for some laboratories in the table?
Some laboratories had no vitamin D entry on their price lists on this site, so no price could be shown for them without inventing one. Aga Khan University Hospital is shown without a number because its individual test prices are not publicly listed; confirm the price with the hospital directly.
Full Laboratory Price Lists
For the complete test lists used in this comparison, see: Chughtai Lab test list, Dr. Essa Lab test list, IDC test list, Dow Lab test list, Ibn-E-Sina Clinical Laboratory test list, Alnoor Diagnostic Centre test list, and Khan Lab test list.
All Lab Tests is an independent price-comparison website and is not affiliated with, or endorsed by, any of the laboratories named above. Prices were taken from each laboratory’s price list on this site, as listed in November 2026, except for the Chughtai Lab price, where the official Chughtai Lab rate is used and the difference is noted above. Prices may vary by branch or city and can change without notice, so confirm the current price before booking. This article is for general information only and is not medical advice; for diagnosis, treatment or interpretation of any test result, consult a qualified doctor.
